Pyrophosphate-condensing activity linked to nucleic acid synthesis
نویسندگان
چکیده
منابع مشابه
Synthesis and biological properties of triazole-linked locked nucleic acid.
We have synthesized and studied the biological and biophysical properties of triazole-linked ribo and xylo locked nucleic acid (LNA). The combination of LNA with the Isobe triazole linkage gave high binding affinity when incorporated at the 3' or 5' termini of oligonucleotides, but low binding affinity at internal positions. Antisense oligonucleotides (ASOs) and siRNAs containing triazole dimer...
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An unnatural alpha-D-mannopyranose-linked chitobiosyl dolichyl pyrophosphate, a stereoisomer of the N-glycan biosynthesis intermediate, was synthesized. The protected trisaccharide, alpha-D-Man-(1-->4)-beta-D-GlcNAc-(1-->4)-D-GlcNAc, carrying a 4-methylbenzoyl group was prepared for the convenience of a TLC analysis. 1-O-Phosphorylation, condensation with dolichyl phosphate, and subsequent depr...
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Background: One of the remaining questions in the understanding of the origin of Nature’s information system is the way the first nucleic acids have been synthesized. This could have been realized using nucleoside triphosphates or imidazolides of nucleoside monophosphates as building blocks. Alternatively, dinucleoside pyrophosphates could have been used for this purpose. The advantage of using...
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A new, general preparation of 2'-5'-linked oligoadenylates has been developed, which relies on: (1) chemoselective 0phosphorylation of N-unblocked nucleosides via hydroxyl activation by Grignard reagents, (2) one-pot construction of internucleotide linkage using bifunctional phosphorylating agents, and (3) selective production of a 3',5'-di-O-protected adenosine. This solution-phase synthesis a...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1979
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/6.4.1521